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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human BDNF protein- GMP grade

EC50

4-20 ng/mL

Specific Activity

minimally 3.00 x105 IU/mg, typically 4.55 x105 IU/mg

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1335-GMP

Print datasheet

Synonyms

Abrineurin, ANON2, brain-derived neurotrophic factor

Validation Data Gallery

  • Purity of recombinant human BDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human BDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP BDNF (HZ-1335-GMP) stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 4-20 ng/mL.

    GMP BDNF (HZ-1335-GMP) stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 4-20 ng/mL.

Technical Specifications

GeneID 627
Species Human
Expression HEK293
EC50 4-20 ng/mL
Specific Activityminimally 3.00 x105 IU/mg, typically 4.55 x105 IU/mg
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP23560
Molecular Mass 14 kDa reduced and non-reduced, homodimer, non-glycosylated
Formulation 10 x PBS pH 7.4 See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family, which also includes NGF, NT-3, NT-4, and NT-6. It plays a critical role in the survival, differentiation, and maturation of neurons during development. It is primarily expressed in various regions of the brain including the hippocampus, amygdala, cerebellum, and cerebral cortex. BDNF binds with the TrkB receptor, which results in the activation of downstream pathways controlling neural plasticity, neurogenesis, and stress resistance. It may also play a role in promoting angiogenesis and endothelial cell survival. Decreased levels of BDNF have been associated with several neurodegenerative diseases such as Parkinson’s, multiple sclerosis, and Huntington’s disease.

Synonyms

Abrineurin, ANON2, brain-derived neurotrophic factor