• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

uPAR, PLAUR Recombinant antibody, PBS Only

uPAR, PLAUR Recombinant Antibody for FC, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230148A1

Cat No : 82953-1-PBS

Print datasheet

Synonyms

CD87, MO3, PLAUR, U PAR, UPAR, URKR

验证数据展示

  • FC experiment of A431 using 82953-1-RR (same clone as 82953-1-PBS)

    FC experiment of A431 using 82953-1-RR (same clone as 82953-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human uPAR, PLAUR (82953-1-RR, Clone: 230148A1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

    IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR, PLAUR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

    IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR, PLAUR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

产品信息

82953-1-PBS targets uPAR, PLAUR in FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen uPAR, PLAUR fusion protein Ag28056 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PLAUR
Synonyms CD87, MO3, PLAUR, U PAR, UPAR, URKR
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC002788
Gene Symbol PLAUR
Gene ID (NCBI) 5329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03405
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)