IP result of anti-MECP2 (IP:10861-1-AP, 4ug; Detection:10861-1-AP 1:500) with HEK-293T cells lysate 1640 ug.

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

1X10^6 NIH/3T3 cells were stained with 0.2ug MECP2 antibody (10861-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MECP2 antibody (10861-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MECP2 antibody (10861-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®488 MECP2 antibody (CL488-10861) at dilution of 1:100, CL594-Phalloidin (red).

1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug CoraLite®488 Anti-Human MECP2 (CL488-10861) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug CoraLite®555 Anti-Human MECP2 (CL555-10861) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 MECP2 antibody (CL555-10861) at dilution of 1:100.

MECP2 fusion protein Ag29632 SDS-PAGE

MeCP2 phospho Ser80 antibody (pAb) tested by Western blot. Detection of MeCP2 phospho Ser80 by Western blot analysis. Rat brain extract (20 ug) probed with MeCP2 phospho Ser80 antibody (pAb) at a 1:5,000 dilution.