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COXIV Monoclonal antibody, PBS Only (Capture)

COXIV Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A7B2

Cat no : 66110-1-PBS

Print datasheet

Synonyms

COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1, 2A7B2

验证数据展示

  • WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

    WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

    IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

    IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

    WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50551-3

    Cytometric bead array standard curve of MP50551-3

    Cytometric bead array standard curve of MP50551-3, COXIV Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66110-1-PBS. Detection antibody: 60251-4-PBS. Standard:Ag1640. Range: 1.563-200 ng/mL.

产品信息

66110-1-PBS targets COXIV as part of a matched antibody pair:

MP50551-3: 66110-1-PBS capture and 60251-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen COXIV fusion protein Ag20551 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome c oxidase subunit IV isoform 1
Synonyms COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1, 2A7B2
Calculated Molecular Weight19.6 kDa
Observed Molecular Weight 17-18 kDa
GenBank Accession NumberBC021236
Gene Symbol COX IV
Gene ID (NCBI) 1327
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13073
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.