Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®488 CD31 antibody (CL488-66065, Clone: 2A1E2 ) at dilution of 1:400.
1X10^6 THP-1 cells were surface stained with 0.4 ug CoraLite®488 Anti-Human CD31 (CL488-66065, Clone:3F8E2) (red), or 0.4 ug Control Antibody. Cells were not fixed.
