Various lysates were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP result of anti-ENDOG (IP:22148-1-AP, 4ug; Detection:22148-1-AP 1:1000) with mouse heart tissue lysate 4000ug.