Various lysates were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB result of PTEN antibody (22034-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HEK-293T cells.
IP result of anti-PTEN (IP:22034-1-AP, 4ug; Detection:22034-1-AP 1:500) with DU 145 cells lysate 1800 ug.
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PTEN (22034-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .