Immunoprecipitation of mCherry fusion with RFP-Trap.I: Input, FT: Flow-through, B: Bound.

RFP-Trap Magnetic Agarose for immunoprecipitation of RFP and mCherry fusion proteins.I: Input, FT: Flow-through, B: Bound.

RFP-Trap Magnetic Agarose for immunoprecipitation of RFP and mCherry fusion proteins.I: Input, FT: Flow-through, B: Bound.

Immunoprecipitation of mCherry fusion with RFP-Trap.I: Input, FT: Flow-through, B: Bound.

Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

Make the most of your time & gain about 40 hours with the iST_RFP-Trap Kit.

The HA-Trap Agarose Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

Co-IP using HA-Trap Agarose Kit followed by multiplexed WB of TOM70-HA and HSP90 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with full-length TOM70-HA construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. TOM70 Monoclonal Antibody (66593-1-Ig), Multi-rAB CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM002), HSP90 Polyclonal Antibody (13171-1-AP), and Multi-rAb CoraLite Plus 750-Goat Anti Rabbit Recombinant Secondary Antibody (RGAR006) were used in the WB analysis.