Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-PAF49 labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, yellow), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta) and DAPI (blue).
Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and DAPI (blue).
Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta), CoraLite® 594-conjugated GNL3 antibody (CL594-67169) and DAPI (blue).
Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), mouse anti-Lamin and anti-mouse IgG secondary antibody Alexa Fluor® 568 (red), rabbit anti-COXIV (11242-1-AP) and anti-rabbit IgG secondary antibody Alexa Fluor® 647 (cyan) and DAPI (blue).
Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green). Cell nuclei are in blue.
Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 7505 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041).
WB of HeLa and HepG2 cell lysates: HeLa and HepG2 cell lysates were detected with anti-HSP90 (60318-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-CACYBP (68161-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-TUFM (67802-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue).