AbFlex ATM phospho Ser1981 antibody (rAb) tested by Western blot. 20 ug HeLa nuclear extract (high salt preparation) untreated (lane 1) or treated with VP16 at 100 uM for 8 hrs (Lane 2), probed with 1 ug/ml ATM phospho Ser1981 antibody. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
AbFlex ATM phospho Ser1981 antibody specifity Dot blot analysis was used to confirm the specificity of ATM phospho Ser1981 antibody. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with antibody (0.5 ug/ml dilution). 1: ATM phospho serine 1981 (SLAFEEG(Sph)QSTTISS) 2: H2AX phospho serine 139 (CKATQA[Sph]QEY) 3: ATM unmodified serine 1981 (SLAFEEGSQSTTISS) 4: NBS phospho serine 343 (CPGPSL[Sph]QGVS) 5: BRCA1 phospho serine 1423 (LEQHG[Sph]QSNSC)
