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ARF1 Polyclonal antibody

ARF1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat no : 20226-1-AP

Print datasheet

Synonyms

EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1

验证数据展示

WB analysis using 20226-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20226-1-AP (ARF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20226-1-AP
WB result of ARF1 antibody (20226-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HEK-293 cells.
IP experiment of HeLa using 20226-1-AP
IP result of anti-ARF1 (IP:20226-1-AP, 3ug; Detection:20226-1-AP 1:600) with HeLa cells lysate 2440ug.
IHC staining of human breast cancer using 20226-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARF1 antibody included
IHC staining of human breast cancer using 20226-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARF1 antibody included
IF Staining of HepG2 using 20226-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20226-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 20226-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARF1 (20226-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 20226-1-AP

Size

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经过测试的应用

Positive WB detected in	DU 145 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, SKOV-3 cells, MDA -MB-231 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human brain tissue, human colon cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy ARF1 Ready-To-Use IHC Kit. ARF1 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 8 publications below
IF	See 2 publications below
IP	See 1 publications below
CoIP	See 1 publications below

产品信息

20226-1-AP targets ARF1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IF, IP, CoIP
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Immunogen	Peptide 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	ADP-ribosylation factor 1
Synonyms	EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	18-21 kDa
GenBank Accession Number	NM_001658
Gene Symbol	ARF1
Gene ID (NCBI)	375
RRID	AB_2878659
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P84077
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.

实验方案

Product Specific Protocols
WB protocol for ARF1 antibody 20226-1-AP	Download protocol
IHC protocol for ARF1 antibody 20226-1-AP	Download protocol
IF protocol for ARF1 antibody 20226-1-AP	Download protocol
IP protocol for ARF1 antibody 20226-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nat Immunol Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense. Authors - Miwa Sasai View Article
human,mouse	WB	Nat Commun Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation Authors - Mutian Jia View Article
human	WB	Nat Commun ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling Authors - Maximilian Hirschenberger View Article
human	WB	PLoS Pathog Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. Authors - Tao Wang View Article
human	WB,IF	J Cell Sci The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response. Authors - Yuanyuan Su View Article
human	WB	Sci Rep In vivo monitoring of the recruitment and activation of AP-1 by Arf1. Authors - Etienne Sauvageau View Article

ARF1 Polyclonal antibody

ARF1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

验证数据展示

WB analysis using 20226-1-AP

WB analysis of HEK-293 using 20226-1-AP

IP experiment of HeLa using 20226-1-AP

IHC staining of human breast cancer using 20226-1-AP

IHC staining of human breast cancer using 20226-1-AP

IF Staining of HepG2 using 20226-1-AP

FC experiment of HeLa using 20226-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response.

In vivo monitoring of the recruitment and activation of AP-1 by Arf1.

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Flow Cytometry Perm Buffer (10X)