• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0

Flow Cytometry Perm Buffer (10X)

Concentration:

10x

Application:

FC

Cat No : PF00011-C

Validation Data Gallery

  • 1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed with 1X Transcription Factor Fix/Perm buffer (PF00011-A) and permeabilized with 1X Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).<br>

    1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed with 1X Transcription Factor Fix/Perm buffer (PF00011-A) and permeabilized with 1X Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).

Product Information

Flow Cytometry Perm Buffer (10X) Provided as a concentrate which, when diluted with distilled water to a 1X solution, provide best results during intracellular staining of cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and washing steps.

Storage

4°C

Expiration Date

6 months after receiving.