ATG16L1 Polyclonal antibody
ATG16L1 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, MCF-7 cells, Jurkat cells, HEK-293T cells |
| Positive IP detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 19812-1-AP is ATG16L1 Fusion Protein expressed in E. coli.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | CoIP, IF, IHC, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | ATG16L1 fusion protein Ag13844 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ATG16 autophagy related 16-like 1 (S. cerevisiae) |
| Synonyms | APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, IBD10, WDR30 |
| Calculated Molecular Weight | 607 aa, 68 kDa |
| Observed Molecular Weight | 63-71 kDa |
| GenBank Accession Number | BC000061 |
| Gene Symbol | ATG16L1 |
| Gene ID (NCBI) | 55054 |
| RRID | AB_10695631 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q676U5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG16L1 antibody 19812-1-AP | Download protocol |
| IP protocol for ATG16L1 antibody 19812-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1 | ||
Nat Commun Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates. | ||
Autophagy AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability. | ||
Curr Biol Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy. | ||
Cancer Lett Let-7i-5p promotes a malignant phenotype in nasopharyngeal carcinoma via inhibiting tumor-suppressive autophagy. | ||
Clin Transl Immunology Circular RNA TRAPPC6B inhibits intracellular Mycobacterium tuberculosis growth while inducing autophagy in macrophages by targeting microRNA-874-3p. |
