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ATP6V1E2 Polyclonal antibody

ATP6V1E2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13235-1-AP

Print datasheet

Synonyms

ATP6E1, ATP6EL2, ATP6V1E2, ATP6V1EL2, V ATPase subunit E 2, VMA4

验证数据展示

  • WB analysis of mouse brain using 13235-1-AP

    WB analysis of mouse brain using 13235-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13235-1-AP (ATP6V1E2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 13235-1-AP

    IP experiment of mouse brain using 13235-1-AP

    IP result of anti-ATP6V1E2 (IP:13235-1-AP, 4ug; Detection:13235-1-AP 1:1000) with mouse brain tissue lysate 3000 ug.

经过测试的应用

Positive WB detected inmouse brain tissue, MCF-7 cells
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13235-1-AP targets ATP6V1E2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATP6V1E2 fusion protein Ag4088 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2
Synonyms ATP6E1, ATP6EL2, ATP6V1E2, ATP6V1EL2, V ATPase subunit E 2, VMA4
Calculated Molecular Weight 226 aa, 26 kDa
Observed Molecular Weight 31-33 kDa
GenBank Accession NumberBC034808
Gene Symbol ATP6V1E2
Gene ID (NCBI) 90423
RRIDAB_2062675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP6V1E2 antibody 13235-1-APDownload protocol
IP protocol for ATP6V1E2 antibody 13235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols