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BDH1 Polyclonal antibody
BDH1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, mouse liver tissue, rat liver tissue, HT-29 cells, human liver tissue, rat brain tissue, COLO 320 cells, mouse brain tissue, mouse heart tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | rat brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse liver tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15417-1-AP targets BDH1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | BDH1 fusion protein Ag7705 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | 3-hydroxybutyrate dehydrogenase, type 1 |
Synonyms | Short chain dehydrogenase/reductase family 9C member 1, SDR9C1, EC:1.1.1.30, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH |
Calculated Molecular Weight | 38 kDa |
Observed Molecular Weight | 31 kDa |
GenBank Accession Number | BC005844 |
Gene Symbol | BDH1 |
Gene ID (NCBI) | 622 |
RRID | AB_2274683 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q02338 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for BDH1 antibody 15417-1-AP | Download protocol |
IHC protocol for BDH1 antibody 15417-1-AP | Download protocol |
IF protocol for BDH1 antibody 15417-1-AP | Download protocol |
IP protocol for BDH1 antibody 15417-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
J Cell Biol CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. |