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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BDH1 Polyclonal antibody

BDH1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15417

Print datasheet

Synonyms

Short chain dehydrogenase/reductase family 9C member 1, SDR9C1, EC:1.1.1.30, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH

验证数据展示

  • FC experiment of HepG2 using CL488-15417

    FC experiment of HepG2 using CL488-15417

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human BDH1 (CL488-15417) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BDH1 Ready-To-Use IHC Kit. BDH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15417-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15417 targets BDH1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen BDH1 fusion protein Ag7705 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 3-hydroxybutyrate dehydrogenase, type 1
Synonyms Short chain dehydrogenase/reductase family 9C member 1, SDR9C1, EC:1.1.1.30, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC005844
Gene Symbol BDH1
Gene ID (NCBI) 622
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02338
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 BDH1 antibody CL488-15417Download protocol
Standard Protocols
Click here to view our Standard Protocols