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CoraLite® Plus 488-conjugated Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC (Intra) and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-30000

Print datasheet

Synonyms

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

验证数据展示

  • FC experiment of HeLa using CL488-30000

    FC experiment of HeLa using CL488-30000

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Rabbit IgG control (CL488-30000) (red), or 0.2 ug CoraLite® Plus 488-conjugated Galectin-3 Rabbit PolyAb (CL488-14979) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-30000 targets Rabbit IgG control in IF, FC (Intra) applications and shows reactivity with n/a samples.

Tested Applications FC (Intra) Application Description
Cited ApplicationsIF
Tested Reactivity n/a
Immunogen N/A 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rabbit IgG control
Synonyms Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2919216
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Rabbit IgG control antibody CL488-30000Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell

A pan-cancer single-cell panorama of human natural killer cells

Authors - Fei Tang
View Article