Rabbit IgG control Polyclonal antibody
Rabbit IgG control Polyclonal Antibody for FC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Applications
FC, IP, WB, ELISA and More (6)
Conjugate
Unconjugated
254
验证数据展示
经过测试的应用
| Positive WB detected in | Protein, Control |
| Positive IP detected in | Control |
| Positive FC detected in | Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30000-0-AP targets Rabbit IgG control in WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA, Cell treatment applications and shows reactivity with samples.
| Tested Applications | FC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, Cell treatment |
| Tested Reactivity | |
| Cited Reactivity | human, mouse, rat, sheep, bovine |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Rabbit IgG control |
| Synonyms | IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin, RIGGCON |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2819035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
发表文章
| Species | Application | Title |
|---|---|---|
Drug Resist Updat CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis | ||
Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity | ||
ACS Nano Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling | ||
Mol Cell CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity | ||
Mol Cell Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy. |
