BrdU Monoclonal antibody
BrdU Monoclonal Antibody for IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat and More (1)
Applications
IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B10E12
验证数据展示
经过测试的应用
Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, L-929 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66241-1-Ig targets BrdU in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, IF/ICC, ELISA Application Description |
Cited Applications | IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish |
Immunogen | Compound 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | BrdU |
Synonyms | 5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2881630 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protocol for IHC:
https://www.ptgcn.com/protocol/66241-1-IgIHC.pdf
Protocol for IF:
https://www.ptgcn.com/protocol/66241-1-IgIF.pdf
实验方案
Product Specific Protocols | |
---|---|
IHC protocol for BrdU antibody 66241-1-Ig | Download protocol |
IF protocol for BrdU antibody 66241-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab The browning and mobilization of subcutaneous white adipose tissue supports efficient skin repair | ||
Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis. | ||
Sci Transl Med FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis. | ||
Cell Res A novel m6A reader Prrc2a controls oligodendroglial specification and myelination. | ||
Nat Commun Replication stress triggers microsatellite destabilization and hypermutation leading to clonal expansion in vitro. |