BrdU Monoclonal antibody

BrdU Monoclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1B10E12

Cat no : 66241-1-Ig

Print datasheet

Synonyms

5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR



经过测试的应用

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, L-929 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66241-1-Ig targets BrdU in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Immunogen Compound 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name BrdU
Synonyms 5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881630
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has 0mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

Protocol for IHC:
https://www.ptgcn.com/protocol/66241-1-IgIHC.pdf

Protocol for IF:
https://www.ptgcn.com/protocol/66241-1-IgIF.pdf

实验方案

Product Specific Protocols
IHC protocol for BrdU antibody 66241-1-IgDownload protocol
IF protocol for BrdU antibody 66241-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Metab

The browning and mobilization of subcutaneous white adipose tissue supports efficient skin repair

Authors - Junrong Cai
mouseIHC,IF

Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu
mouseIF

Sci Transl Med

FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis.

Authors - Tokio Matsuzaki
mouseIF

Cell Res

A novel m6A reader Prrc2a controls oligodendroglial specification and myelination.

Authors - Rong Wu
humanIF

Nat Commun

MRNIP condensates promote DNA double-strand break sensing and end resection.

Authors - Yun-Long Wang
mouseIF

Nat Commun

Replication stress triggers microsatellite destabilization and hypermutation leading to clonal expansion in vitro.

Authors - Yusuke Matsuno