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验证数据展示

  • IF Staining of L-929 using 66241-1-Ig (same clone as 66241-1-PBS)

    IF Staining of L-929 using 66241-1-Ig (same clone as 66241-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed L-929 cells using BrdU antibody (66241-1-Ig, Clone: 1B10E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66241-1-Ig (same clone as 66241-1-PBS)

    IF Staining of HeLa using 66241-1-Ig (same clone as 66241-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 66241-1-Ig (BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 66241-1-Ig (same clone as 66241-1-PBS)

    IHC staining of mouse spleen using 66241-1-Ig (same clone as 66241-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66241-1-Ig (same clone as 66241-1-PBS)

    IF Staining of HeLa using 66241-1-Ig (same clone as 66241-1-PBS)

    Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using 66241-1-Ig(BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

BrdU Monoclonal antibody, PBS Only

BrdU Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 66241-1-PBS
CloneNo. 1B10E12

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

BUdR, Bromodeoxyuridine, BrdUrd, 5-bromo-2-deoxyuridine

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66241-1-PBS targets BrdU in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Compound 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name BrdU
Synonyms BUdR, Bromodeoxyuridine, BrdUrd, 5-bromo-2-deoxyuridine
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881630
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has 0mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

Protocol for IHC:
https://www.ptgcn.com/protocol/66241-1-IgIHC.pdf

Protocol for IF:
https://www.ptgcn.com/protocol/66241-1-IgIF.pdf