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  • KD/KO Validated

Cyclin D2 Polyclonal antibody

Cyclin D2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10934-1-AP

Print datasheet

Synonyms

CCND2, cyclin D, cyclin D2, G1/S-specific cyclin-D2



经过测试的应用

Positive WB detected inCaco-2 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, HEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10934-1-AP targets Cyclin D2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, bovine, goat
Immunogen Cyclin D2 fusion protein Ag1377 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin D2
Synonyms CCND2, cyclin D, cyclin D2, G1/S-specific cyclin-D2
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC010958
Gene Symbol Cyclin D2
Gene ID (NCBI) 894
RRIDAB_2275319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

实验方案

Product Specific Protocols
WB protocol for Cyclin D2 antibody 10934-1-APDownload protocol
IHC protocol for Cyclin D2 antibody 10934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Nature

SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma.

Authors - Soufiane Boumahdi
humanWB

Cancer Cell

The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation.

Authors - Natalia Martinez-Soria
  • KD Validated
humanWB

Mol Cancer

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Authors - Xi Jin
mouseWB

Dev Cell

Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.

Authors - Yan Gong
mouse

Kidney Int

Transcription factor MafB may play an important role in secondary hyperparathyroidism.

Authors - Naoki Morito
mouseWB

Ecotoxicol Environ Saf

Exposure to nicotine regulates prostaglandin E2 secretion and autophagy of granulosa cells to retard follicular maturation in mammals

Authors - Xiaofeng Zhou