CD14 Polyclonal antibody
CD14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig and More (2)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, human spleen tissue, RAW 264.7 cells, pig spleen tissue |
Positive IHC detected in | human hepatocirrhosis tissue, human appendicitis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17000-1-AP targets CD14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, pig |
Cited Reactivity | human, mouse, rat, pig, sheep |
Immunogen | CD14 fusion protein Ag10693 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD14 molecule |
Synonyms | Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule |
Calculated Molecular Weight | 375 aa, 40 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC010507 |
Gene Symbol | CD14 |
Gene ID (NCBI) | 929 |
ENSEMBL Gene ID | ENSG00000170458 |
RRID | AB_2074048 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08571 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD14 antibody 17000-1-AP | Download protocol |
IHC protocol for CD14 antibody 17000-1-AP | Download protocol |
IF protocol for CD14 antibody 17000-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Immunol Clostridium septicum α-toxin activates the NLRP3 inflammasome by engaging GPI-anchored proteins. | ||
Nat Commun HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis. | ||
EBioMedicine Patient-derived melanoma organoid models facilitate the assessment of immunotherapies | ||
Biomaterials Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury | ||
Biomaterials Cerebro- and renoprotective activities through platelet-derived biomaterials against cerebrorenal syndrome in rat model. |