CD14 Polyclonal antibody

CD14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17000-1-AP

Print datasheet

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule



经过测试的应用

Positive WB detected inHL-60 cells, human spleen tissue, RAW 264.7 cells, pig spleen tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human appendicitis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17000-1-AP targets CD14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse, rat, pig, sheep
Immunogen CD14 fusion protein Ag10693 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD14 molecule
Synonyms Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_2074048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08571
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
WB protocol for CD14 antibody 17000-1-APDownload protocol
IHC protocol for CD14 antibody 17000-1-APDownload protocol
IF protocol for CD14 antibody 17000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Br J Sports Med

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

Authors - Stephanie Georgina Dakin
mouseWB

Sci Immunol

Clostridium septicum α-toxin activates the NLRP3 inflammasome by engaging GPI-anchored proteins.

Authors - Weidong Jing
human,mouse,ratFC

Nat Commun

HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis.

Authors - Chao Liang
humanIF

EBioMedicine

Patient-derived melanoma organoid models facilitate the assessment of immunotherapies

Authors - Lingling Ou
mouseIF

Biomaterials

Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury

Authors - Xin Liu
ratIF

Biomaterials

Cerebro- and renoprotective activities through platelet-derived biomaterials against cerebrorenal syndrome in rat model.

Authors - Hon-Kan Yip