CoraLite® Plus 488-conjugated CD14 Polyclonal antibody
CD14 Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF-P detected in | human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17000-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-17000 targets CD14 in IF-P applications and shows reactivity with human samples.
Tested Applications | IF-P Application Description |
Tested Reactivity | human |
Immunogen | CD14 fusion protein Ag10693 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD14 molecule |
Synonyms | CD14, CD14 molecule |
Calculated Molecular Weight | 375 aa, 40 kDa |
GenBank Accession Number | BC010507 |
Gene Symbol | CD14 |
Gene ID (NCBI) | 929 |
ENSEMBL Gene ID | ENSG00000170458 |
RRID | AB_2919143 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08571 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CD14 antibody CL488-17000 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |