验证数据展示
经过测试的应用
| Positive WB detected in | unboiled A431 cells, 37°C incubated A549 cells, unboiled human placenta tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CD151 Ready-To-Use IHC Kit. CD151 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66567-1-Ig targets CD151 in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | CD151 fusion protein Ag25985 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD151 molecule (Raph blood group) |
| Synonyms | CD151, CD151 antigen, GP27, Membrane glycoprotein SFA 1, MER2, PETA 3, RAPH, SFA1, Tetraspanin 24, Tspan 24, TSPAN24 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC001374 |
| Gene Symbol | CD151 |
| Gene ID (NCBI) | 977 |
| RRID | AB_2881928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P48509 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD151 antibody 66567-1-Ig | Download protocol |
| IHC protocol for CD151 antibody 66567-1-Ig | Download protocol |
| IP protocol for CD151 antibody 66567-1-Ig | Download protocol |
| FC protocol for CD151 antibody 66567-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Downregulation of CD151 induces oxidative stress and apoptosis in trophoblast cells via inhibiting ERK/Nrf2 signaling pathway in preeclampsia.
| ||
PLoS One Downregulation of the CD151 protects the cardiac function by the crosstalk between the endothelial cells and cardiomyocytes via exosomes | ||
Stem Cells Transl Med Factor 3 regulates airway engraftment by human bronchial basal cells |