验证数据展示
经过测试的应用
Positive IHC detected in | human liver tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
IF | See 2 publications below |
产品信息
66779-1-Ig targets CD16 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, IF-P, ELISA Application Description |
Cited Applications | IHC, IF |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | CD16 fusion protein Ag9787 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
Synonyms | Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A |
Calculated Molecular Weight | 254 aa, 29 kDa |
GenBank Accession Number | BC017865 |
Gene Symbol | CD16a |
Gene ID (NCBI) | 2214 |
ENSEMBL Gene ID | ENSG00000203747 |
RRID | AB_2918477 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08637 |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.
实验方案
Product Specific Protocols | |
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IHC protocol for CD16 antibody 66779-1-Ig | Download protocol |
IF protocol for CD16 antibody 66779-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Oncol IL-2 Combined with IL-15 Enhanced the Expression of NKG2D Receptor on Patient Autologous NK Cells to Inhibit Wilms' Tumor via MAPK Signaling Pathway | ||
Front Immunol Neoadjuvant immunochemotherapy improves clinical outcomes of patients with esophageal cancer by mediating anti-tumor immunity of CD8+ T (Tc1) and CD16+ NK cells |