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CD16 Monoclonal antibody, PBS Only

CD16 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E12E11

Cat no : 66779-1-PBS

Print datasheet

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

验证数据展示

  • IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD16 antibody included
  • IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD16 antibody included
  • IF Staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

    IF Staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD16 antibody (66779-1-Ig, Clone: 1E12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

产品信息

66779-1-PBS targets CD16 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen CD16 fusion protein Ag9787 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Synonyms Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC017865
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.