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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD16 antibody included
  • IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD16 antibody included
  • IHC staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

    IHC staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD16 antibody included
  • IF Staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

    IF Staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD16 antibody (66779-1-Ig, Clone: 1E12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

CD16 Monoclonal antibody, PBS Only

CD16 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 66779-1-PBS

产品说明书

CloneNo. 1E12E11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, IF-P, ELISA

别名

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

产品信息

66779-1-PBS targets CD16 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 CD16 fusion protein Ag9787 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
计算分子量 254 aa, 29 kDa
GenBank蛋白编号BC017865
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
RRIDAB_2918477
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08637
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.