验证数据展示
经过测试的应用
Positive IHC detected in | human liver tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
IF | See 2 publications below |
产品信息
66779-1-Ig targets CD16 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, IF-P, ELISA Application Description |
文献引用应用 | IHC, IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | CD16 fusion protein Ag9787 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
别名 | Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A |
计算分子量 | 254 aa, 29 kDa |
GenBank蛋白编号 | BC017865 |
基因名称 | CD16a |
Gene ID (NCBI) | 2214 |
ENSEMBL Gene ID | ENSG00000203747 |
RRID | AB_2918477 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P08637 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.
实验方案
Product Specific Protocols | |
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IHC protocol for CD16 antibody 66779-1-Ig | Download protocol |
IF protocol for CD16 antibody 66779-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Oncol IL-2 Combined with IL-15 Enhanced the Expression of NKG2D Receptor on Patient Autologous NK Cells to Inhibit Wilms' Tumor via MAPK Signaling Pathway | ||
Front Immunol Neoadjuvant immunochemotherapy improves clinical outcomes of patients with esophageal cancer by mediating anti-tumor immunity of CD8+ T (Tc1) and CD16+ NK cells |