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CKB Monoclonal antibody

CKB Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7C3

Cat no : 66764-1-Ig

Print datasheet

Synonyms

B CK, CKB, CKBB, Creatine kinase B chain, Creatine kinase B type, creatine kinase, brain



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CKB Ready-To-Use IHC Kit. CKB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66764-1-Ig targets CKB in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen CKB fusion protein Ag7933 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name creatine kinase, brain
Synonyms B CK, CKB, CKBB, Creatine kinase B chain, Creatine kinase B type, creatine kinase, brain
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001190
Gene Symbol CKB
Gene ID (NCBI) 1152
RRIDAB_2882110
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12277
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

实验方案

Product Specific Protocols
WB protocol for CKB antibody 66764-1-IgDownload protocol
IHC protocol for CKB antibody 66764-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Theranostics

Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype

Authors - Zhi-Jie Gao
humanWB

Pharmacol Res

Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.

Authors - Jiale Zhu
  • KD Validated
humanWB

Transl Oncol

RNA-binding protein IGF2BP2 suppresses metastasis of clear cell renal cell carcinoma by enhancing CKB mRNA stability and expression

Authors - Junwu Ren