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CLRN1 Polyclonal antibody

CLRN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26630-1-AP

Print datasheet

Synonyms

clarin 1, CLRN1, USH3, USH3A, Usher syndrome type 3 protein

验证数据展示

  • IHC staining of mouse testis using 26630-1-AP

    IHC staining of mouse testis using 26630-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26630-1-AP (CLRN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 26630-1-AP

    IHC staining of mouse testis using 26630-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26630-1-AP (CLRN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 26630-1-AP

    WB analysis of HeLa using 26630-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26630-1-AP (CLRN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26630-1-AP targets CLRN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CLRN1 fusion protein Ag24289 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clarin 1
Synonyms clarin 1, CLRN1, USH3, USH3A, Usher syndrome type 3 protein
Calculated Molecular Weight 232 aa, 26 kDa
Observed Molecular Weight 23 kDa, 27 kDa
GenBank Accession NumberBC074970
Gene Symbol CLRN1
Gene ID (NCBI) 7401
RRIDAB_2880579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLRN1 antibody 26630-1-APDownload protocol
IHC protocol for CLRN1 antibody 26630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

J Pathol

Clarin-1 expression in adult mouse and human retina highlights a role of Müller glia in Usher syndrome.

Authors - Lei Xu
View Article