验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12393-1-AP targets COPG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | COPG fusion protein Ag3070 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coatomer protein complex, subunit gamma |
| 别名 | Gamma-1-COP, Gamma-1-coat protein, COPG1, Coatomer subunit gamma-1 |
| 计算分子量 | 874 aa, 98 kDa |
| 观测分子量 | 98 kDa |
| GenBank蛋白编号 | BC020498 |
| 基因名称 | COPG |
| Gene ID (NCBI) | 22820 |
| RRID | AB_2081435 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y678 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COPG antibody 12393-1-AP | Download protocol |
| IHC protocol for COPG antibody 12393-1-AP | Download protocol |
| IF protocol for COPG antibody 12393-1-AP | Download protocol |
| IP protocol for COPG antibody 12393-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Deficiency in coatomer complex I causes aberrant activation of STING signalling. | ||
J Exp Med Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation. | ||
J Biol Chem Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice. | ||
Hum Mol Genet Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. | ||
