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验证数据展示

  • WB analysis using 28404-1-AP

    WB analysis using 28404-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28404-1-AP (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28404-1-AP

    IHC staining of human breast cancer using 28404-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28404-1-AP (CUL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CUL1 antibody included
  • IHC staining of human breast cancer using 28404-1-AP

    IHC staining of human breast cancer using 28404-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28404-1-AP (CUL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CUL1 antibody included

CUL1 Polyclonal antibody

CUL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28404-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

CUL 1, CUL1, cullin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CUL1 Ready-To-Use IHC Kit. CUL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28404-1-AP targets CUL1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 CUL1 fusion protein Ag29145 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cullin 1
别名 CUL 1, CUL1, cullin 1
计算分子量 90 kDa
观测分子量 80 kDa
GenBank蛋白编号BC034318
基因名称 CUL1
Gene ID (NCBI) 8454
RRIDAB_2881135
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13616
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

实验方案

Product Specific Protocols
WB protocol for CUL1 antibody 28404-1-APDownload protocol
IHC protocol for CUL1 antibody 28404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols