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EIF2A/CDA02 Monoclonal antibody
EIF2A/CDA02 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1G7H4
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, ROS1728 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
66482-1-Ig targets EIF2A/CDA02 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human |
Immunogen | EIF2A/CDA02 fusion protein Ag22041 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | eukaryotic translation initiation factor 2A, 65kDa |
Synonyms | CDA02, EIF 2A, EIF2A, EIF2A/CDA02, MST089, MSTP004, MSTP089 |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC011885 |
Gene Symbol | EIF2A |
Gene ID (NCBI) | 83939 |
RRID | AB_2881848 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9BY44 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
IHC protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
IF protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Pharm Biol Cinobufagin induces acute promyelocytic leukaemia cell apoptosis and PML-RARA degradation in a caspase-dependent manner by inhibiting the β-catenin signalling pathway | ||
Front Neurosci Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease |