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验证数据展示

  • IF Staining of HepG2 using CL555-66482

    IF Staining of HepG2 using CL555-66482

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®555 EIF2A/CDA02 antibody (CL555-66482, Clone: 1G7H4 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated EIF2A/CDA02 Monoclonal antibody

EIF2A/CDA02 Monoclonal Antibody for IF/ICC
Cat No. CL555-66482

Print datasheet

CloneNo. 1G7H4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

CDA02, EIF 2A, EIF2A, MST089, MSTP004, MSTP089

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66482-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66482 targets EIF2A/CDA02 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen EIF2A/CDA02 fusion protein Ag22041 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2A, 65kDa
Synonyms CDA02, EIF 2A, EIF2A, MST089, MSTP004, MSTP089
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC011885
Gene Symbol EIF2A
Gene ID (NCBI) 83939
RRIDAB_2934674
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BY44
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.

实验方案

Product Specific Protocols
IF protocol for CL555 EIF2A/CDA02 antibody CL555-66482Download protocol
Standard Protocols
Click here to view our Standard Protocols