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Factor X Monoclonal antibody

Factor X Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3D3F11

Cat no : 66753-1-Ig

Print datasheet

Synonyms

coagulation factor X, F10, Factor X, FX, FXA, Stuart factor, Stuart Prower factor

验证数据展示

  • WB analysis of human plasma using 66753-1-Ig

    WB analysis of human plasma using 66753-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66753-1-Ig (Factor X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66753-1-Ig

    IHC staining of human liver using 66753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 66753-1-Ig

    IHC staining of human liver using 66753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver using 66753-1-Ig

    IF Staining of human liver using 66753-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Factor X antibody (66753-1-Ig, Clone: 3D3F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66753-1-Ig targets Factor X in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human
Immunogen Factor X fusion protein Ag11664 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coagulation factor X
Synonyms coagulation factor X, F10, Factor X, FX, FXA, Stuart factor, Stuart Prower factor
Calculated Molecular Weight 488 aa, 55 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC046125
Gene Symbol F10
Gene ID (NCBI) 2159
RRIDAB_2882099
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00742
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

实验方案

Product Specific Protocols
WB protocol for Factor X antibody 66753-1-IgDownload protocol
IHC protocol for Factor X antibody 66753-1-IgDownload protocol
IF protocol for Factor X antibody 66753-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols