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验证数据展示

  • IF Staining of human liver using CL488-66753

    IF Staining of human liver using CL488-66753

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite® Plus 488 Factor X antibody (CL488-66753, Clone: 3D3F11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Factor X Monoclonal antibody

Factor X Monoclonal Antibody for IF-P
Cat No. CL488-66753

Print datasheet

CloneNo. 3D3F11

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Synonyms

coagulation factor X, F10, FX, FXA, Stuart factor, Stuart Prower factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver tissue
For other applications, we recommend the unconjugated version of this antibody, 66753-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66753 targets Factor X in IF-P applications and shows reactivity with Human samples.

Tested Applications IF-P Application Description
Tested Reactivity Human
Immunogen Factor X fusion protein Ag11664 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coagulation factor X
Synonyms coagulation factor X, F10, FX, FXA, Stuart factor, Stuart Prower factor
Calculated Molecular Weight 488 aa, 55 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC046125
Gene Symbol F10
Gene ID (NCBI) 2159
RRIDAB_2934494
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00742
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Factor X antibody CL488-66753Download protocol
Standard Protocols
Click here to view our Standard Protocols