验证数据展示

WB analysis using 16276-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 16276-1-AP
Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (16276-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
WB analysis of HepG2 using 16276-1-AP
WB result of G3BP2 antibody (16276-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP2 transfected HepG2 cells.
IHC staining of human lung cancer using 16276-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with G3BP2 antibody included
IP experiment of HeLa using 16276-1-AP
IP result of anti-G3BP2 (IP:16276-1-AP, 4ug; Detection:16276-1-AP 1:1000) with HeLa cells lysate 2200 ug.
IHC staining of human lung cancer using 16276-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with G3BP2 antibody included
WB analysis using 16276-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Featured Product
KD/KO Validated

G3BP2 Polyclonal antibody

G3BP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16276-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Synonyms

G3BP 2, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2, G3BP-2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®555

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (25)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	A549 cells, HepG2 cells, MCF-7 cells, T47D cells, HeLa cells, Jurkat cells, HEK-293 cells, Neuro-2a cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, mouse liver tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	sodium arsenite treated HeLa cells

Planning an IHC experiment? We recommend our IHCeasy G3BP2 Ready-To-Use IHC Kit. G3BP2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 19 publications below
IHC	See 3 publications below
IF	See 11 publications below
IP	See 5 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

产品信息

16276-1-AP targets G3BP2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, RIP
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Immunogen	G3BP2 fusion protein Ag9355 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	GTPase activating protein (SH3 domain) binding protein 2
Synonyms	G3BP 2, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2, G3BP-2
Calculated Molecular Weight	482aa,54 kDa; 449aa,51 kDa
Observed Molecular Weight	55-60 kDa
GenBank Accession Number	BC011731
Gene Symbol	G3BP2
Gene ID (NCBI)	9908
RRID	AB_2878237
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UN86
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

实验方案

Product Specific Protocols
WB protocol for G3BP2 antibody 16276-1-AP	Download protocol
IHC protocol for G3BP2 antibody 16276-1-AP	Download protocol
IF protocol for G3BP2 antibody 16276-1-AP	Download protocol
IP protocol for G3BP2 antibody 16276-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,CoIP	Cell Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules Authors - Qinqin Cui View Article
human		Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Authors - Peiguo Yang View Article
human	WB	Cell Rep hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility Authors - Xiaoli Wang View Article
human	WB,IP	Oncogene HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer Authors - Bei Liu KD Validated View Article
human	WB,IF,IP	Oncogene RIOK1 mediates p53 degradation and radioresistance in colorectal cancer through phosphorylation of G3BP2. Authors - Yaqi Chen KD Validated View Article
human	WB,IHC	Mol Cancer Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis. Authors - Hongwei Liu KD Validated View Article

验证数据展示

WB analysis using 16276-1-AP

IF Staining of HeLa using 16276-1-AP

WB analysis of HepG2 using 16276-1-AP

IHC staining of human lung cancer using 16276-1-AP

IP experiment of HeLa using 16276-1-AP

IHC staining of human lung cancer using 16276-1-AP

WB analysis using 16276-1-AP

G3BP2 Polyclonal antibody

G3BP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer

RIOK1 mediates p53 degradation and radioresistance in colorectal cancer through phosphorylation of G3BP2.

Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.