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CoraLite®555-conjugated G3BP2 Polyclonal antibody

G3BP2 Polyclonal Antibody for IF/ICC
Cat No. CL555-16276

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

G3BP 2, G3BP2, KIAA0660

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected insodium arsenite treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy G3BP2 Ready-To-Use IHC Kit. G3BP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16276-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-16276 targets G3BP2 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 G3BP2 fusion protein Ag9355 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTPase activating protein (SH3 domain) binding protein 2
别名 G3BP 2, G3BP2, KIAA0660
计算分子量482aa,54 kDa; 449aa,51 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC011731
基因名称 G3BP2
Gene ID (NCBI) 9908
RRIDAB_2919631
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UN86
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

实验方案

Product Specific Protocols
IF protocol for CL555 G3BP2 antibody CL555-16276Download protocol
Standard Protocols
Click here to view our Standard Protocols
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