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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated G3BP2 Polyclonal antibody

G3BP2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-16276

Print datasheet

Synonyms

G3BP 2, G3BP2, KIAA0660

验证数据展示

  • IF Staining of HeLa using CL555-16276

    IF Staining of HeLa using CL555-16276

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®555 G3BP2 antibody (CL555-16276) at dilution of 1:200.

  • IF Staining of HeLa using CL555-16276

    IF Staining of HeLa using CL555-16276

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®555 G3BP2 antibody (CL555-16276) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected insodium arsenite treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy G3BP2 Ready-To-Use IHC Kit. G3BP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16276-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL555-16276 is G3BP2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen G3BP2 fusion protein Ag9355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTPase activating protein (SH3 domain) binding protein 2
Synonyms G3BP 2, G3BP2, KIAA0660
Calculated Molecular Weight482aa,54 kDa; 449aa,51 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC011731
Gene Symbol G3BP2
Gene ID (NCBI) 9908
RRIDAB_2919631
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN86
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

实验方案

Product Specific Protocols
IF protocol for CL555 G3BP2 antibody CL555-16276Download protocol
Standard Protocols
Click here to view our Standard Protocols