HBB Polyclonal antibody
HBB Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, human placenta tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human appendicitis tissue, human placenta tissue |
| Positive FC (Intra) detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy HBB Ready-To-Use IHC Kit. HBB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IHC | See 3 publications below |
产品信息
16216-1-AP targets HBB in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | HBB fusion protein Ag9216 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hemoglobin, beta |
| Synonyms | Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin |
| Calculated Molecular Weight | 147 aa, 16 kDa |
| Observed Molecular Weight | 13-16 kDa |
| GenBank Accession Number | BC007075 |
| Gene Symbol | HBB |
| Gene ID (NCBI) | 3043 |
| RRID | AB_10598329 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P68871 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HBB antibody 16216-1-AP | Download protocol |
| IHC protocol for HBB antibody 16216-1-AP | Download protocol |
| IF protocol for HBB antibody 16216-1-AP | Download protocol |
| IP protocol for HBB antibody 16216-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Elife ARID1A loss in adult hepatocytes activates β-catenin-mediated erythropoietin transcription. | ||
Stem Cells Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells. | ||
Blood Cells Mol Dis A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression. |
