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验证数据展示

  • FC experiment of K-562 using CL750-16216

    FC experiment of K-562 using CL750-16216

    1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human HBB (CL750-16216) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human appendicitis using CL750-16216

    IF Staining of human appendicitis using CL750-16216

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 750 HBB antibody (CL750-16216) at dilution of 1:200.

CoraLite® Plus 750-conjugated HBB Polyclonal antibody

HBB Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL750-16216

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Synonyms

Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750

Size: 
100 μL

¥3200/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive IF-P detected inhuman appendicitis tissue
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy HBB Ready-To-Use IHC Kit. HBB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16216-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-16216 targets HBB in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen HBB fusion protein Ag9216 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hemoglobin, beta
Synonyms Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin
Calculated Molecular Weight 147 aa, 16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC007075
Gene Symbol HBB
Gene ID (NCBI) 3043
RRIDAB_3673690
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68871
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.

实验方案

Product Specific Protocols
IF protocol for CL Plus 750 HBB antibody CL750-16216Download protocol
Standard Protocols
Click here to view our Standard Protocols