验证数据展示
经过测试的应用
Positive IF-P detected in | human appendicitis tissue |
Positive FC (Intra) detected in | K-562 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-16216 targets HBB in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | IF-P, FC (Intra) Application Description |
Tested Reactivity | human, mouse |
Immunogen | HBB fusion protein Ag9216 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | hemoglobin, beta |
Synonyms | Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin |
Calculated Molecular Weight | 147 aa, 16 kDa |
Observed Molecular Weight | 13-16 kDa |
GenBank Accession Number | BC007075 |
Gene Symbol | HBB |
Gene ID (NCBI) | 3043 |
RRID | AB_3673690 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P68871 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 750 HBB antibody CL750-16216 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |