验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, human placenta tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human appendicitis tissue, human placenta tissue |
| Positive FC (Intra) detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16216-1-AP targets HBB in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | HBB fusion protein Ag9216 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hemoglobin, beta |
| 别名 | Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin |
| 计算分子量 | 147 aa, 16 kDa |
| 观测分子量 | 13-16 kDa |
| GenBank蛋白编号 | BC007075 |
| 基因名称 | HBB |
| Gene ID (NCBI) | 3043 |
| RRID | AB_10598329 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P68871 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HBB antibody 16216-1-AP | Download protocol |
| IHC protocol for HBB antibody 16216-1-AP | Download protocol |
| IF protocol for HBB antibody 16216-1-AP | Download protocol |
| IP protocol for HBB antibody 16216-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Elife ARID1A loss in adult hepatocytes activates β-catenin-mediated erythropoietin transcription. | ||
Stem Cells Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells. | ||
Blood Cells Mol Dis A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression. |
