HMGB1 Monoclonal antibody
HMGB1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse and More (1)
Applications
WB, ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
1D12A6
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, L02 cells, K-562 cells |
Positive IF-P detected in | mouse lung tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66525-1-Ig targets HMGB1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB, IHC, IP, CoIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | HMGB1 fusion protein Ag1264 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | high-mobility group box 1 |
Synonyms | HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 30 kDa |
GenBank Accession Number | BC003378 |
Gene Symbol | HMGB1 |
Gene ID (NCBI) | 3146 |
RRID | AB_2881888 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P09429 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HMGB1 antibody 66525-1-Ig | Download protocol |
IF protocol for HMGB1 antibody 66525-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Microbiol Chloroquine ameliorates carbon tetrachloride-induced acute liver injury in mice via the concomitant inhibition of inflammation and induction of apoptosis | ||
Adv Healthc Mater Anti-PD-L1 DNAzyme Loaded Photothermal Mn 2+ /Fe 3+ Hybrid Metal-Phenolic Networks for Cyclically Amplified Tumor Ferroptosis-Immunotherapy | ||
J Control Release Enhancing cell pyroptosis with biomimetic nanoparticles for melanoma chemo-immunotherapy | ||
ACS Appl Mater Interfaces Cascade-Responsive Hierarchical Nanosystems for Multisite Specific Drug Exposure and Boosted Chemoimmunotherapy | ||
J Nanobiotechnology Glioma-targeted oxaliplatin/ferritin clathrate reversing the immunosuppressive microenvironment through hijacking Fe2+ and boosting Fenton reaction | ||
Redox Biol Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma. |