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  • Featured Product
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HPRT1 Polyclonal antibody

HPRT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15059-1-AP

Print datasheet

Synonyms

Hypoxanthine-guanine phosphoribosyltransferase, HPRT, HGPRTase, HGPRT, EC:2.4.2.8

验证数据展示

  • WB analysis using 15059-1-AP

    WB analysis using 15059-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 15059-1-AP

    IP experiment of mouse brain using 15059-1-AP

    IP result of anti-HPRT1 (IP:15059-1-AP, 4ug; Detection:15059-1-AP 1:6000) with mouse brain tissue lysate 1280 ug.

  • IF Staining of HeLa using 15059-1-AP

    IF Staining of HeLa using 15059-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HPRT1 antibody (15059-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human brain using 15059-1-AP

    IHC staining of human brain using 15059-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of human brain using 15059-1-AP

    IHC staining of human brain using 15059-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with HPRT1 antibody included
  • FC experiment of HeLa using 15059-1-AP

    FC experiment of HeLa using 15059-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HPRT1 (15059-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HEK-293 using 15059-1-AP

    WB analysis of HEK-293 using 15059-1-AP

    WB result of HPRT1 antibody (15059-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT1 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, mouse liver tissue, MCF-7 cells, HepG2 cells, Jurkat cells, Y79 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human pancreas tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HPRT1 Ready-To-Use IHC Kit. HPRT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15059-1-AP targets HPRT1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen HPRT1 fusion protein Ag7044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hypoxanthine phosphoribosyltransferase 1
Synonyms Hypoxanthine-guanine phosphoribosyltransferase, HPRT, HGPRTase, HGPRT, EC:2.4.2.8
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC000578
Gene Symbol HPRT1
Gene ID (NCBI) 3251
RRIDAB_10638622
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00492
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPRT1, also named HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout.

实验方案

Product Specific Protocols
WB protocol for HPRT1 antibody 15059-1-APDownload protocol
IHC protocol for HPRT1 antibody 15059-1-APDownload protocol
IF protocol for HPRT1 antibody 15059-1-APDownload protocol
IP protocol for HPRT1 antibody 15059-1-APDownload protocol
FC protocol for HPRT1 antibody 15059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Invest

Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.

Authors - Xiaoyan Yang
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ratWB

J Clin Invest

ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice.

Authors - Hidemichi Kouzu
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mouseWB

Cell Rep

Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways

Authors - Tatsuki Yasuda
View Article
human,mouseWB

Br J Cancer

Blocking of oestrogen signals improves anti-tumour effect regardless of oestrogen receptor alpha expression in cancer cells

Authors - Nabeel Kajihara
View Article
humanWB

EMBO Rep

MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.

Authors - Gongwei Wu
View Article
mouseWB

Elife

Aging is associated with increased brain iron through cortex-derived hepcidin expression.

Authors - Tatsuya Sato
View Article