human IgG heavy chain Polyclonal antibody

human IgG heavy chain Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 16402-1-AP

Print datasheet

Synonyms

Human IgG4, IgG Fc, IGHG4, IGHG, IgG4



经过测试的应用

Positive WB detected inhuman plasma tissue, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Human IgG4 Ready-To-Use IHC Kit. Human IgG4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16402-1-AP targets human IgG heavy chain in WB, IHC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IP, CoIP, RIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen human IgG heavy chain fusion protein Ag9519 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Synonyms Human IgG4, IgG Fc, IGHG4, IGHG, IgG4
Calculated Molecular Weight 473 aa, 52 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC025985
Gene Symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2878254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01861
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGHG4 has some fragments with MW 36 kDa (http://www.uniprot.org/uniprot/P01861) and 52 kDa (http://www.ncbi.nlm.nih.gov/protein/CAE45900). This antibody detects all the Heavy chain of human immunoglobulin gamma (IgG), IgG1, IgG2, IgG3, IgG4 included.

实验方案

Product Specific Protocols
WB protocol for human IgG heavy chain antibody 16402-1-APDownload protocol
IHC protocol for human IgG heavy chain antibody 16402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Nat Commun

Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia.

Authors - Franc Llorens
humanIHC

Neuropathol Appl Neurobiol

Plasma total prion protein as a potential biomarker for neurodegenerative dementia: diagnostic accuracy in the spectrum of prion diseases.

Authors - Franc Llorens
humanCoIP

Mol Oncol

Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer.

Authors - Chao Fang
human,mouseWB,IP

Cancer Gene Ther

miR-451a was selectively sorted into exosomes and promoted the progression of esophageal squamous cell carcinoma through CAB39

Authors - Lu Wang
humanRIP

J Dent Sci

Circ_0004491 stimulates guanine nucleotide-binding protein alpha subunit to inhibit the malignant progression of oral squamous cell carcinoma by sponging miR-2278

Authors - Tao Cheng
WB

Oncol Rep

Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells.

Authors - Li Liu