IHCeasy Human IgG4 Ready-To-Use IHC Kit
Human IgG4 Ready-to-use reagent kit for IHC.
Reactivity
Human
Sample Type
FFPE tissue
Cat No : KHC0074
Synonyms
Human IgG4, IgG4, IGHG4
Validation Data Gallery
Product Information
KHC0074 is a ready-to-use IHC kit for staining of Human IgG4. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy Human IgG4 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Serum IgG4 is a subtype of IgG, and its levels are frequently elevated and particularly high in autoimmune pancreatitis (AIP) as well as other disease. It's now a common concept that IgG4 related disease (IgG4-RD) is a systemic immune-mediated fibro-inflammatory condition often involving several organ systems, especially pancreas and salivary glands. A serum IgG4 cut off level of 135mg/mL was considered a reliable marker predictive of IgG4-RD. IgG4 positive plasma cell infiltration is also ubiquitous in IgG4-RD.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | Human IgG4, IgG4, IGHG4 |