IgG Kappa Light Chain Polyclonal antibody

IgG Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA and More (5)

Conjugate

Unconjugated

Cat no : 14678-1-AP

Print datasheet

Synonyms

IgG Kappa Light Chain, IGK, IGK-, IGKC, immunoglobulin kappa locus



经过测试的应用

Positive WB detected inhuman plasma tissue, human liver tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman spleen tissue, human heart tissue, human placenta tissue, human skin tissue, human testis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14678-1-AP targets IgG Kappa Light Chain in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, monkey
Immunogen IgG Kappa Light Chain fusion protein Ag6389 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin kappa locus
Synonyms IgG Kappa Light Chain, IGK, IGK-, IGKC, immunoglobulin kappa locus
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC030813
Gene Symbol IgG Kappa Light Chain
Gene ID (NCBI) 50802
RRIDAB_1851235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the light chain (Kappa) of human IgG.

实验方案

Product Specific Protocols
WB protocol for IgG Kappa Light Chain antibody 14678-1-APDownload protocol
IHC protocol for IgG Kappa Light Chain antibody 14678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Nat Commun

A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.

Authors - Christine Quach
IHC

PLoS Genet

Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis.

Authors - Hyun-Jin Na
humanRIP

J Bone Miner Metab

Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes.

Authors - Lanjuan Shen
mouseRIP

Mol Med Rep

Long non‑coding RNA XIST promotes cerebral ischemia/reperfusion injury by modulating miR‑27a‑3p/FOXO3 signaling.

Authors - Hua Zhang
mouseRIP

J Exp Clin Cancer Res

RNA N6-methyladenosine reader IGF2BP2 promotes lymphatic metastasis and epithelial-mesenchymal transition of head and neck squamous carcinoma cells via stabilizing slug mRNA in an m6A-dependent manner.

Authors - Dan Yu
humanIP

Heliyon

HMGA2 overexpression activates IGF2BP2 to stabilize APLP2 via m6A modification and promote pancreatic cancer progression

Authors - Ke Liu