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验证数据展示

  • WB analysis of Ramos using 66829-1-Ig

    WB analysis of Ramos using 66829-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66829-1-Ig

    WB analysis using 66829-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 66829-1-Ig

    WB analysis of Jurkat using 66829-1-Ig

    WB result of IL-12RB1 antibody (66829-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12RB1 transfected Jurkat cells.

  • IF Staining of THP-1 using 66829-1-Ig

    IF Staining of THP-1 using 66829-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL-12RB1 antibody (66829-1-Ig, Clone: 1E10E10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO验证

IL-12RB1 Monoclonal antibody

IL-12RB1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66829-1-Ig

产品说明书

CloneNo. 1E10E10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

IL12RB1, IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inRamos cells, TF-1 cells, THP-1 cells, Jurkat cells, Raji cells, K-562 cells, Daudi cells
Positive IF/ICC detected inTHP-1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66829-1-Ig targets IL-12RB1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 IL-12RB1 fusion protein Ag6920 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 12 receptor, beta 1
别名 IL12RB1, IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1
计算分子量 662 aa, 73 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC029121
基因名称 IL12RB1
Gene ID (NCBI) 3594
RRIDAB_2882172
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP42701
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

实验方案

Product Specific Protocols
WB protocol for IL-12RB1 antibody 66829-1-IgDownload protocol
IF protocol for IL-12RB1 antibody 66829-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols