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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IL-12RB1 Monoclonal antibody

IL-12RB1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E10E10

Cat no : CL488-66829

Print datasheet

Synonyms

IL12RB1, IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1

验证数据展示

  • FC experiment of K-562 using CL488-66829

    FC experiment of K-562 using CL488-66829

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-12RB1 (CL488-66829, Clone:1E10E10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 66829-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66829 targets IL-12RB1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen IL-12RB1 fusion protein Ag6920 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 12 receptor, beta 1
Synonyms IL12RB1, IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1
Calculated Molecular Weight 662 aa, 73 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC029121
Gene Symbol IL12RB1
Gene ID (NCBI) 3594
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP42701
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-12RB1 antibody CL488-66829Download protocol
Standard Protocols
Click here to view our Standard Protocols