IL-1RA Polyclonal antibody
IL-1RA Polyclonal Antibody for WB, IF/ICC, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, FC, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells |
Positive IHC detected in | human oesophagus tissue, human colon tissue, human colon cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells |
Positive FC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
10844-1-AP targets IL-1RA in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, FC, IHC, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | IL-1RA fusion protein Ag1277 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | interleukin 1 receptor antagonist |
Synonyms | Anakinra, ICIL 1RA, IL 1ra, IL 1ra3, IL 1RN, IL1 inhibitor, IL1F3, IL1RA, IL-1RA, IL1RN, IRAP |
Calculated Molecular Weight | 20 kDa |
Observed Molecular Weight | 18-20 kDa |
GenBank Accession Number | BC009745 |
Gene Symbol | IL1RN |
Gene ID (NCBI) | 3557 |
RRID | AB_2125559 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P18510 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for IL-1RA antibody 10844-1-AP | Download protocol |
IHC protocol for IL-1RA antibody 10844-1-AP | Download protocol |
IF protocol for IL-1RA antibody 10844-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Clin Transl Oncol Inhibitory effect and mechanism of mesenchymal stem cells on melanoma cells. | ||
NeuroImmune Pharm Ther Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages | ||
Front Immunol The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy |