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  • Featured Product
  • KD/KO Validated

IQGAP1 Polyclonal antibody

IQGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22167-1-AP

Print datasheet

Synonyms

Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01

验证数据展示

  • WB analysis using 22167-1-AP

    WB analysis using 22167-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22167-1-AP

    IHC staining of human breast cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IHC staining of human breast cancer using 22167-1-AP

    IHC staining of human breast cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IP experiment of HeLa using 22167-1-AP

    IP experiment of HeLa using 22167-1-AP

    IP result of anti-IQGAP1 (IP:22167-1-AP, 4ug; Detection:22167-1-AP 1:8000) with HeLa cells lysate 1085 ug.

  • IF Staining of A549 using 22167-1-PBS

    IF Staining of A549 using 22167-1-PBS

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A549 using 22167-1-AP

    IF Staining of A549 using 22167-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 22167-1-AP

    IF Staining of HeLa using 22167-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22167-1-AP (IQGAP1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 22167-1-AP

    FC experiment of HeLa using 22167-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IQGAP1 (22167-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human normal colon using 22167-1-AP

    IHC staining of human normal colon using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IF Staining of MCF-7 using 22167-1-AP

    IF Staining of MCF-7 using 22167-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inA549 cells, NIH/3T3 cells, SGC-7901 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A549 cells, MCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IQGAP1 Ready-To-Use IHC Kit. IQGAP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22167-1-AP targets IQGAP1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen IQGAP1 fusion protein Ag17821 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IQ motif containing GTPase activating protein 1
Synonyms Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01
Calculated Molecular Weight 189 kDa
Observed Molecular Weight 190-195 kDa
GenBank Accession NumberBC151834
Gene Symbol IQGAP1
Gene ID (NCBI) 8826
ENSEMBL Gene IDENSG00000140575
RRIDAB_10949193
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46940
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

实验方案

Product Specific Protocols
WB protocol for IQGAP1 antibody 22167-1-APDownload protocol
IHC protocol for IQGAP1 antibody 22167-1-APDownload protocol
IF protocol for IQGAP1 antibody 22167-1-APDownload protocol
IP protocol for IQGAP1 antibody 22167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma

Authors - Perla Pucci
View Article
humanWB,IP

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
  • KD Validated
View Article
humanWB

Theranostics

Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.

Authors - Hui-Fang Hu
View Article
WB

Oncogene

The scaffold protein IQGAP1 links heat-induced stress signals to alternative splicing regulation in gastric cancer cells.

Authors - Andrada-Maria Birladeanu
  • KO Validated
View Article
humanWB

Oncotarget

Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1.

Authors - Hyeongsun Moon
View Article
humanWB,IF

Front Cell Infect Microbiol

IQGAP1 Mediates Hcp1-Promoted Escherichia coli Meningitis by Stimulating the MAPK Pathway.

Authors - Mingna Zhao
  • KD Validated
View Article