验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy IQGAP1 Ready-To-Use IHC Kit. IQGAP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22167-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-22167 targets IQGAP1 in IF/ICC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | IQGAP1 fusion protein Ag17821 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | IQ motif containing GTPase activating protein 1 |
| 别名 | Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01 |
| 计算分子量 | 189 kDa |
| 观测分子量 | 190-195 kDa |
| GenBank蛋白编号 | BC151834 |
| 基因名称 | IQGAP1 |
| Gene ID (NCBI) | 8826 |
| ENSEMBL Gene ID | ENSG00000140575 |
| RRID | AB_3084064 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46940 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 IQGAP1 antibody CL488-22167 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |